An in vitro synthetic biosystem based on acetate for production of phloroglucinol

نویسندگان

  • Rubing Zhang
  • Wei Liu
  • Yujin Cao
  • Xin Xu
  • Mo Xian
  • Huizhou Liu
چکیده

BACKGROUND Phloroglucinol is an important chemical, and the biosynthesis processes which can convert glucose to phloroglucinol have been established. However, due to approximate 80% of the glucose being transformed into undesirable by-products and biomass, this biosynthesis process only shows a low yield with the highest value of about 0.20 g/g. The industrial applications are usually hindered by the low current productivity and yield and also by the high costs. Generally, several different aspects limit the development of phloroglucinol biosynthesis. The yield of phloroglucinol is one of the most important parameters for its bioconversion especially from economic and ecological points of view. The in vitro biosynthesis of bio-based chemicals, is a flexible alternative with potentially high-yield to in vivo biosynthetic technology. RESULTS By comparing the activity of acetyl-CoA synthetase (ACS) from Escherichia coli and Acetobacter pasteurianus, the highly active ACS2 was identified in A. pasteurianus. Acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus and phloroglucinol synthase (PhlD) from Pseudomonas fluorescens pf-5 were expressed and purified. Acetate was successfully transformed into phloroglucinol by the combined activity of above-mentioned enzymes and required cofactor. After optimization of the in vitro reaction system, phloroglucinol was then produced with a yield of nearly 0.64 g phloroglucinol/g acetic acid, which was equal to 91.43% of the theoretically possible maximum. CONCLUSIONS In this work, a novel in vitro synthetic system for a highly efficient production of phloroglucinol from acetate was demonstrated. The system's performance suggests that in vitro synthesis of phloroglucinol has some advantages and is potential to become a feasible industrial alternative. Based on the results presented herewith, it is believed that in vitro biosystem will provide a feasible option for production of important industrial chemicals from acetate, which could work as a versatile biosynthetic platform.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Phloroglucinol and Silver Nitrate Enhances Axillary Shoot Proliferation in Nodal Explants of Vitex negundo L. –an Aromatic Medicinal Plant

A reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant Vitex negundo through in vitro culture. Of different concentrations of N6-benzyladenine (BA) evaluated as supplements to Murahige and Skoog (MS) medium, BA at 1mg/l was effective in enhancing bud break and shoot regeneration. Response of explants was al...

متن کامل

In vitro Assessment of Adsorbents to Counteract Lead Toxicity in Ruminal Fermentation

An in vitro gas production technique was used to evaluate the effects of different adsorbents on the gas production parameters of lead-exposed diet. Ruminal fluid, obtained from fistulated sheep (3-4 years of age and 40±5 kg BW), and experimental diets samples, 200 ± 0.2 mg DM, were incubated in 100 mL glass bottles. To each bottle, one of the following treatments was applied: 1) control (no Pb...

متن کامل

Effect of Sweeteners on Viscosity and Particle Size of Dilute Guar Gum Solutions

The effects of some synthetic sweeteners on the rheological and physical properties of guar gum in dilute solutions were investigated.Measurements include the determination of intrinsic viscosity and the particle size, surface weighted mean [D3, 2], volume weighted mean [D4,3] and specificsurface area ofguar gum andsyntheticsweeteners mixtures. T...

متن کامل

Optimizing Energy Consumption Efficiency for Greenhouse Cucumber Production Using The Data Envelopment Analysis Technique in Lorestan Province of Iran

This paper applied a non-parametric (Data Envelopment Analysis) method to analyze the efficiency of farmers, discriminate efficient farmers from inefficient ones and to identify wasteful uses of energy in order to optimize the energy inputs for greenhouse cucumber production in Lorestan province of Iran. Data were collected from 27 cucumber producers by using a face-to-face questionnaire.DEA cr...

متن کامل

Optimizing Energy Consumption Efficiency for Greenhouse Cucumber Production Using The Data Envelopment Analysis Technique in Lorestan Province of Iran

This paper applied a non-parametric (Data Envelopment Analysis) method to analyze the efficiency of farmers, discriminate efficient farmers from inefficient ones and to identify wasteful uses of energy in order to optimize the energy inputs for greenhouse cucumber production in Lorestan province of Iran. Data were collected from 27 cucumber producers by using a face-to-face questionnaire.DEA cr...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 17  شماره 

صفحات  -

تاریخ انتشار 2017